Antioxidant and Enzyme Inhibitory Properties, and HPLC-MS/MS Profiles of Different Extracts of Arabis carduchorum Boiss.: An Endemic Plant to Turkey
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Date
2022
Authors
Uba, Abdullahi Ibrahim
Zengin, Gokhan
Montesano, Domenico
Cakilcioglu, Ugur
Selvi, Selami
Ulusan, Musa Denizhan
Caprioli, Giovanni
Journal Title
Journal ISSN
Volume Title
Publisher
Mdpi
Abstract
In this study, six extracts of Arabis carduchorum prepared from solvents of varying polarity (water, methanol, ethyl acetate, dichloromethane, and n-hexane) were investigated for their chemical profiles and total phenolic and flavonoid contents (TPC/TFC) using HPLC-MS/MS and spectrophotometric/colorimetric assays, respectively, along with the assessment of their antioxidant and enzyme inhibitory effects. In general, the polar extracts (methanol, water, and infusion) showed higher TPC/TFC than the other extracts (range: 13.73-26.62 mg GAE/g; 2.66-52.95 mg RE/g, respectively). The total antioxidant capacity of the polar extracts ranged from 0.90-1.85 mmol TE/g in phosphomolybdenum assay, while the free radical scavenging potential ranged from 4.36-32.13 mg TE/g and 12.58-67.73 mg TE/g in DPPH and ABTS assays, respectively. While the water and infuse extract inhibited only butyrylcholinesterase (2.68 and 2.39 mg GALAE/g, respectively), the other extracts were found to inhibit both acetyl- and butyryl-cholinesterases (0.96-2.73 mg GALAE/g and 1.86-5.11 mg GALAE/g, respectively). On the contrary, all extracts exhibited anti-tyrosinase (40.24-59.22 mg KAE/g) and anti-amylase (0.09-0.63 mmol ACAE/g) activities. Overall, this study highlighted the effect of solvent polarity on the extraction of bioactive compounds and the biological properties of the extracts of A. carduchorum; hence, the results obtained could be used to guide optimum extraction in future studies.
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Keywords
Accumulation, Alpina, Arabis carduchorum, phenolics, flavonoids, Seeds, HPLC-MS, MS, Accumulation, solvent polarity, Alpina, antioxidant, Seeds, enzyme inhibition
Turkish CoHE Thesis Center URL
Citation
14
WoS Q
N/A
Scopus Q
Q2
Source
Applied Sciences-Basel
Volume
12
Issue
13