Isgor, Pelin KubraAbbasiasl, TaherDas, RituIstif, EminYener, Umut CanBeker, Levent2024-10-152024-10-15202402635-0998https://doi.org/10.1039/d4sd00135dhttps://hdl.handle.net/20.500.12469/6353Istif, Emin/0000-0003-4700-7050Contact lenses offer a simple, cost-effective, and non-invasive method for in situ real-time analysis of various biomarkers. Electro-chemical sensors are integrated into contact lenses for analysis of various biomarkers. However, they suffer from rigid electronic components and connections, leading to eye irritation and biomarker concentration deviation. Here, a flexible and microfluidic integrated paper-based contact lens for colorimetric analysis of glucose was implemented. Facilitating a three-dimensional (3D) printer for lens fabrication eliminates cumbersome cleanroom processes and provides a simple, batch compatible process. Due to the capillary force of the filter paper, the sample was routed to detection chambers inside microchannels, and it allowed further colorimetric detection. The paper-embedded microfluidic contact lens successfully detects glucose down to 2 mM within similar to 10 s. The small dimension of the microfluidic system enables detection of glucose levels as low as 5 mu l. The results show the potential of the presented approach to analyze glucose concentration in a rapid manner. It is demonstrated that the fabricated contact lens can successfully detect glucose levels of diabetic patients. Contact lenses offer a simple, cost-effective, and non-invasive method for in situ real-time analysis of various biomarkers.eninfo:eu-repo/semantics/openAccess[No Keyword Available]ArticleWOS:00130637760000110.1039/d4sd00135d2-s2.0-85203513336N/AQ339247807