Browsing by Author "Das, Ritu"

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Citation - WoS: 0
Citation - Scopus: 0
Microneedles With Interdigitated Electrodes for in Situ Impedimetric Vegf Sensing
(Wiley, 2025) Das, Ritu; Istif, Emin; Cebecioglu, Rumeysa; Ali, Mohsin; Atik, Yasin; Dag, Cagdas; Beker, Levent
Continuous monitoring of protein biomarkers in interstitial fluid (ISF) is essential for improving patient care and outcomes. This study presents a novel electrochemical impedance spectroscopy (EIS) sensor utilizing microneedles (MNs) patterned with interdigitated electrodes for the detection of vascular endothelial growth factor (VEGF). The MNs, are fabricated from flexible polylactide (PLA) using a simple molding technique, followed by metal deposition with an interdigitated pattern to serve as a platform for the EIS sensor. After functionalization with anti-VEGF antibodies, impedance measurements are conducted to detect VEGF levels, demonstrating a significant change in impedance in response to varying concentrations of the target biomarker. The MNs can easily penetrate rat skin by hand, puncturing without mechanical breakage. This innovative approach enhances the sensitivity and specificity of biomarker detection and paves the way for continuous monitoring applications in diagnostics and preventative medicine.
Citation - WoS: 0
Citation - Scopus: 0
Paper Integrated Microfluidic Contact Lens for Colorimetric Glucose Detection
(Royal Soc Chemistry, 2024) Isgor, Pelin Kubra; Istıf, Emın; Abbasiasl, Taher; Das, Ritu; Istif, Emin; Yener, Umut Can; Beker, Levent
Contact lenses offer a simple, cost-effective, and non-invasive method for in situ real-time analysis of various biomarkers. Electro-chemical sensors are integrated into contact lenses for analysis of various biomarkers. However, they suffer from rigid electronic components and connections, leading to eye irritation and biomarker concentration deviation. Here, a flexible and microfluidic integrated paper-based contact lens for colorimetric analysis of glucose was implemented. Facilitating a three-dimensional (3D) printer for lens fabrication eliminates cumbersome cleanroom processes and provides a simple, batch compatible process. Due to the capillary force of the filter paper, the sample was routed to detection chambers inside microchannels, and it allowed further colorimetric detection. The paper-embedded microfluidic contact lens successfully detects glucose down to 2 mM within similar to 10 s. The small dimension of the microfluidic system enables detection of glucose levels as low as 5 mu l. The results show the potential of the presented approach to analyze glucose concentration in a rapid manner. It is demonstrated that the fabricated contact lens can successfully detect glucose levels of diabetic patients. Contact lenses offer a simple, cost-effective, and non-invasive method for in situ real-time analysis of various biomarkers.