Development of an Enzyme Immunoassay for the Determination of Testosterone

dc.authorscopusid 6602457995
dc.authorscopusid 6507806744
dc.authorscopusid 6701649929
dc.authorscopusid 9636812400
dc.authorscopusid 7005667672
dc.contributor.author Güven, B.
dc.contributor.author Güven, Ebru Bilget
dc.contributor.author Özsar, S.
dc.contributor.author Maraşli, N.
dc.contributor.author Maraşli, Ş.
dc.contributor.author Özcan, A.
dc.contributor.other Molecular Biology and Genetics
dc.date.accessioned 2023-10-19T15:05:16Z
dc.date.available 2023-10-19T15:05:16Z
dc.date.issued 2003
dc.department-temp Güven, B., Kafkas Üniversitesi, Veteriner Fakültesi, Fizyoloji Anabilim Dali, Kars, Turkey; Özsar, S., Kadir Has Üniversitesi, Tip Fakültesi, Biyokimya Anabilim Dali, Istanbul, Turkey; Maraşli, N., Kafkas Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dali, Kars, Turkey; Maraşli, Ş., Kafkas Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dali, Kars, Turkey; Özcan, A., Kafkas Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dali, Kars, Turkey en_US
dc.description.abstract In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be <0.01%. The detection limit of the assay was 2.5 pg/well and the working range of the standard curve was 0-20 ng/ml (0-200 pg/well). The recovery was found to be 98.8% after the addition of known amounts of testosterone to plasma samples. The inter-assay coefficient of variation was 12.6%. The described EIA offers a very economical alternative for the routine estimation of testosterone levels. Only minimal laboratory equipment is required and therefore the assay should be especially useful for research in animal science. en_US
dc.identifier.citationcount 0
dc.identifier.endpage 50 en_US
dc.identifier.issn 1300-0128
dc.identifier.issue 1 en_US
dc.identifier.scopus 2-s2.0-0037226314 en_US
dc.identifier.startpage 45 en_US
dc.identifier.uri https://hdl.handle.net/20.500.12469/4784
dc.identifier.volume 27 en_US
dc.identifier.wosquality Q4
dc.khas 20231019-Scopus en_US
dc.language.iso tr en_US
dc.relation.ispartof Turkish Journal of Veterinary and Animal Sciences en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 0
dc.subject Enzyme immunoassay en_US
dc.subject Testosterone en_US
dc.subject acid anhydride en_US
dc.subject hormone antibody en_US
dc.subject horseradish peroxidase en_US
dc.subject steroid en_US
dc.subject testosterone en_US
dc.subject animal experiment en_US
dc.subject antibody production en_US
dc.subject article en_US
dc.subject blood analysis en_US
dc.subject column chromatography en_US
dc.subject conjugation en_US
dc.subject controlled study en_US
dc.subject cost en_US
dc.subject cross reaction en_US
dc.subject enzyme immunoassay en_US
dc.subject equipment en_US
dc.subject immunization en_US
dc.subject nonhuman en_US
dc.subject rabbit en_US
dc.subject research en_US
dc.subject sex hormone determination en_US
dc.subject standard en_US
dc.subject Animalia en_US
dc.subject Armoracia rusticana en_US
dc.subject Oryctolagus cuniculus en_US
dc.title Development of an Enzyme Immunoassay for the Determination of Testosterone en_US
dc.title.alternative Testosteron İ?çin Enzimimmunassay Tekni?inin Geliştirilmesi en_US
dc.type Article en_US
dspace.entity.type Publication
relation.isAuthorOfPublication 29a3046a-0e10-42f4-a54b-6ff546565470
relation.isAuthorOfPublication.latestForDiscovery 29a3046a-0e10-42f4-a54b-6ff546565470
relation.isOrgUnitOfPublication 71ce8622-7449-4a6a-8fad-44d881416546
relation.isOrgUnitOfPublication.latestForDiscovery 71ce8622-7449-4a6a-8fad-44d881416546

Files

Original bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
4784.pdf
Size:
135.97 KB
Format:
Adobe Portable Document Format
Description:
Tam Metin / Full Text