Browsing by Author "Demirci, Hasan"
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Article Citation - WoS: 9Citation - Scopus: 9Cryogenic X-Ray Crystallographic Studies of Biomacromolecules at Turkish Light Source Turkish Delight(Tubitak Scientific & Technological Research Council Turkey, 2023) Atalay, Necati; Akcan, Enver Kamil; Gul, Mehmet; Ayan, Esra; Destan, Ebru; Ertem, Fatma Betuel; Tokay, Nurettin; Demirci, HasanX-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryocrystallography has never been performed in Turkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, Istanbul, Turkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.