Browsing by Author "Yagi, Sakina"

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Citation - WoS: 3
Citation - Scopus: 3
Chemical profile, antiproliferative and antibacterial activities and docking studies of essential oil and hexane fraction of hydrosol from fresh leaf of Plectranthus amboinicus (Lour.) Spreng.
(Pergamon-Elsevier Science Ltd, 2023) Ibrahim, Enas I. E.; Yagi, Sakina; Tzanova, Tzvetomira; Schohn, Herve; Uba, Abdullahi Ibrahim; Zengin, Gokhan
Essential oils are commonly extracted from plants by hydrodistillation in which an aqueous phase called hydrosol is obtained. Like essential oil, this by-product of distillation can be a source of natural bioactive molecules with health benefits. This study aimed at the investigation of the chemical profile, antiproliferative and antibacterial activities of essential oil and hexane fraction of hydrosol from Plectrunthus amboinicus (family Lamiacease) fresh leaf cultivated in Sudan. Essential oil was obtained by hydrodistilation and hexane fraction (HF) was derived from hydrosol by liquid -liquid extraction. Results showed that the phenolic monoterpene thymol (72.9%) was the dominant component of the essential oil while the HF was characterized by the presence of long-chain (n-C29 to n-C68) alkanes (91.4%). The HF showed highest antiproliferative activity against the colon cancer HT29 (IC50 9.2 mu g/mL) and HCT116 (IC50 7.7 mu g/mL) cell lines. The essential oil exerted best antibacterial activity with highest inhibition (22.0-22.3 mm) and minimum inhibitory concentration (31 mu g/mL) against the Gram positive bacteria Bacillus subtilis and Staphylococcus aureus. In silico study showed that the major components of the oil displayed higher docking scores than those present in the HF suggesting an antagonistic effect by other molecules in the essential oil. In conclusion, beside the essential oil, the hydrosol possesses promising bioactive composition suggesting that it could be exploited for further therapeutic applications.
Citation - WoS: 44
Citation - Scopus: 44
Phenolic Profile, Antioxidant and Enzyme Inhibitory Activities of Leaves From Two Cassia and Two Senna Species
(Mdpi, 2022) Omer, Haifa A. A.; Caprioli, Giovanni; Abouelenein, Doaa; Mustafa, Ahmed M.; Uba, Abdullahi Ibrahim; Ak, Gunes; Ozturk, Refiye Beyza; Yagi, Sakina
Several species within the genera Cassia or Senna have a treasure of traditional medicines worldwide and can be a promising source of bioactive molecules. The objective of the present study was to evaluate the phenolic content and antioxidant and enzyme inhibition activities of leaf methanolic extracts of C. fistula L., C. grandis L., S. alexandrina Mill., and S. italica Mill. The two Cassia spp. contained higher total polyphenolic content (42.23-49.75 mg GAE/g) than the two Senna spp., and C. fistula had significantly (p < 0.05) the highest concentration. On the other hand, the Senna spp. showed higher total flavonoid content (41.47-59.24 mg rutin equivalent per g of extract) than that found in the two Cassia spp., and S. alexandrina significantly (p < 0.05) accumulated the highest amount. HPLC-MS/MS analysis of 38 selected bioactive compounds showed that the majority of compounds were identified in the four species, but with sharp variations in their concentrations. C. fistula was dominated by epicatechin (8928.75 mu g/g), C. grandis by kaempferol-3-glucoside (47,360.04 mu g/g), while rutin was the major compound in S. italica (17,285.02 mu g/g) and S. alexandrina (6381.85). The methanolic extracts of the two Cassia species exerted significantly (p < 0.05) higher antiradical activity, metal reducing capacity, and total antioxidant activity than that recorded from the two Senna species' methanolic extracts, and C. fistula displayed significantly (p < 0.05) the highest values. C. grandis significantly (p < 0.05) exhibited the highest metal chelating power. The results of the enzyme inhibition activity showed that the four species possessed anti-AChE activity, and the highest value, but not significantly (p >= 0.05) different from those obtained by the two Cassia spp., was exerted by S. alexandrina. The Cassia spp. exhibited significantly (p < 0.05) higher anti-BChE and anti-Tyr properties than the Senna spp., and C. grandise revealed significantly (p < 0.05) the highest values. C. grandise revealed significantly (p < 0.05) the highest alpha- amylase inhibition, while the four species had more or less the same effect against the alpha-glucosidase enzyme. Multivariate analysis and in silico studies showed that many of the identified phenols may play key roles as antioxidant and enzyme inhibitory properties. Thus, these Cassia and Senna species could be a promising source of natural bioactive agents with beneficial effects for human health.