Development of an enzyme immunoassay for the determination of testosterone

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Date

2003

Authors

Güven, L
Özsar, Semin
Maraşlı, Nalan
Maraşlı, Şaban
Özcan, Ayla

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Scientific Technical Research Council Turkey-Tubitak

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Abstract

In this study a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be <0.01%. The detection limit of the assay was 2.5 pg/well and the working range of the standard curve was 0-20 ng/ml (0-200 pg/well). The recovery was found to be 98.8% after the addition of known amounts of testosterone to plasma samples. The inter-assay coefficient of variation was 12.6%. The described EIA offers a very economical alternative for the routine estimation of testosterone levels. Only minimal laboratory equipment is required and therefore the assay should be especially useful for research in animal science.

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Testosterone, Enzyme immunoassay

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0

WoS Q

Q4

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N/A

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Volume

27

Issue

1

Start Page

45

End Page

50