The modifier effects of chymotrypsin and trypsin enzymes on fluorescence lifetime distribution of "N-(1-pyrenyl)maleimide-bovine serum albumin" complex

dc.contributor.authorÖzyiğit, İbrahim Ethem
dc.contributor.authorKarakuş, Emine
dc.contributor.authorPekcan, Önder
dc.date.accessioned2021-01-01T10:55:55Z
dc.date.available2021-01-01T10:55:55Z
dc.date.issued2016
dc.departmentFakülteler, Mühendislik ve Doğa Bilimleri Fakültesi, Biyoinformatik ve Genetik Bölümüen_US
dc.description.abstractChymotrypsin and trypsin are the well known proteolytic enzymes, both of which are synthesized in the pancreas as their precursors the inactive forms; chymotrypsinogen and trypsinogen and then are released into the duodenum to cut proteins into smaller peptides. In this paper, the effects of activities of chymotrypsin and trypsin enzymes on fluorescence lifetime distributions of the substrat bovine serum albumin (BSA) modified with N-(1-pyrenyl)maleimide (PM) were examined. In the labeling study of BSA with PM, it is aimed to attach PM to the single free thiol (Cys34) and to all the free amine groups in accessible positions in order to produce excimers of pyrene planes of the possible highest amount to form the lifetime distributions in the widest range, that may show specifically distinguishing changes resulting from the activities of the proteases. The time resolved spectrofluorometer was used to monitor fluorescence decays, which were analyzed by using the exponential series method (ESM) to obtain the changes of lifetime distributions. After the exposure of the synthesized substrat PM-BSA to the enzymes, the fluorescence lifetime distributions exhibited different structures which were attributed to the different activities of the proteases. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.identifier.citation3
dc.identifier.doi10.1016/j.saa.2015.10.008en_US
dc.identifier.endpage12en_US
dc.identifier.issn1386-1425en_US
dc.identifier.issn1386-1425
dc.identifier.pmid26490799en_US
dc.identifier.scopus2-s2.0-84945237149en_US
dc.identifier.scopusqualityQ2
dc.identifier.startpage8en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12469/3696
dc.identifier.urihttps://doi.org/10.1016/j.saa.2015.10.008
dc.identifier.volume154en_US
dc.identifier.wosWOS:000366071200002en_US
dc.identifier.wosqualityN/A
dc.institutionauthorPekcan, Önderen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science LTDen_US
dc.relation.journalSpectrochimica Acta Part A-Molecular and Biomolecular Spectroscopyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFluorescence lifetime distributionen_US
dc.subjectN-(1-pyrenyl)maleimideen_US
dc.subjectExcimeren_US
dc.subjectBovine serum albuminen_US
dc.subjectChymotrypsinen_US
dc.subjectTrypsinen_US
dc.titleThe modifier effects of chymotrypsin and trypsin enzymes on fluorescence lifetime distribution of "N-(1-pyrenyl)maleimide-bovine serum albumin" complexen_US
dc.typeArticleen_US
dspace.entity.typePublication

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