The Gtp-Tubulin Cap Is Not the Determinant of Microtubule End Stability in Cells

dc.authorid Cassidy, Anna/0000-0002-5171-9002
dc.authorid Zanic, Marija/0000-0002-5127-5819
dc.authorscopusid 59348070600
dc.authorscopusid 56378992800
dc.authorscopusid 56091389300
dc.authorscopusid 8540727300
dc.contributor.author Arpağ, Göker
dc.contributor.author Farmer,V.
dc.contributor.author Arpağ,G.
dc.contributor.author Zanic,M.
dc.contributor.other Molecular Biology and Genetics
dc.date.accessioned 2024-10-15T19:42:40Z
dc.date.available 2024-10-15T19:42:40Z
dc.date.issued 2024
dc.department Kadir Has University en_US
dc.department-temp Cassidy A., Department of Cell and Developmental Biology, Vanderbilt University, Nashville, United States; Farmer V., Department of Cell and Developmental Biology, Vanderbilt University, Nashville, United States, Department of Cell Biology, Duke University School of Medicine, Durham, United Kingdom; Arpağ G., Department of Cell and Developmental Biology, Vanderbilt University, Nashville, United States, Department of Molecular Biology and Genetics, Kadir Has University, 34083, Istanbul, Turkey; Zanic M., Department of Cell and Developmental Biology, Vanderbilt University, Nashville, United States, Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, United States, Department of Biochemistry, Vanderbilt University, Nashville, United States en_US
dc.description Cassidy, Anna/0000-0002-5171-9002; Zanic, Marija/0000-0002-5127-5819 en_US
dc.description.abstract Microtubules are dynamic cytoskeletal polymers essential for cell division, motility, and intracellular transport. Microtubule dynamics are characterized by dynamic instability-the ability of individual microtubules to switch between phases of growth and shrinkage. Dynamic instability can be explained by the GTP-cap model, suggesting that a "cap" of GTP-tubulin subunits at the growing microtubule end has a stabilizing effect, protecting against microtubule catastrophe-the switch from growth to shrinkage. Although the GTP-cap is thought to protect the growing microtubule end, whether the GTP-cap size affects microtubule stability in cells is not known. Notably, microtubule end-binding proteins, EBs, recognize the nucleotide state of tubulin and display comet-like localization at growing microtubule ends, which can be used as a proxy for the GTP-cap. Here, we employ high spatiotemporal resolution imaging to compare the relationship between EB comet size and microtubule dynamics in interphase LLC-PK1 cells to that measured in vitro. Our data reveal that the GTP-cap size in cells scales with the microtubule growth rate in the same way as in vitro. However, we find that microtubule ends in cells can withstand transition to catastrophe even after the EB comet is lost. Thus, our findings suggest that the presence of the GTP-cap is not the determinant of microtubule end stability in cells. en_US
dc.description.sponsorship NIH [CA68485, DK58404, EY08126]; Vanderbilt Ingram Cancer Center Resource Share Scholarship [2022-3739440]; National Institutes of Health [T32GM008320, R35GM119552]; American Heart Association Predoctoral Fellowship [19PRE34380083]; Kadir Has University Startup Funds; American Heart Association (AHA) [19PRE34380083] Funding Source: American Heart Association (AHA) en_US
dc.description.sponsorship We thank G. Brouhard (McGill University) , R. Ohi (University of Michigan) , and M. Gardner (University of Minnesota) , for kindly gifting us the XMAP215 construct, LLC-PK1 EB1-GFP cells, and LLC-PK1 tubulin-GFP cells, respectively. We thank the Burnette laboratory (Vanderbilt University) for help with iSIM. All SDC mi-croscopy imaging was performed through the use of the Vanderbilt Cell Imaging Shared Resource (supported by NIH grants CA68485, DK58404, and EY08126) . Use of the core equipment was supported in part by Vanderbilt Ingram Cancer Center Resource Share Scholarship (2022-3739440) . We thank the members of the Zanic laboratory for discussions and feedback. This work was sup-ported by National Institutes of Health grant R35GM119552 to MZ. VF acknowledges support from National Institutes of Health grant T32GM008320 and American Heart Association Predoctoral Fellowship 19PRE34380083. AC acknowledges support from the National Institutes of Health grant T32GM008320. GA acknowledges support from Kadir Has University Startup Funds. The authors de-clare no competing financial interests. en_US
dc.description.woscitationindex Science Citation Index Expanded
dc.identifier.citationcount 0
dc.identifier.doi 10.1091/mbc.E24-07-0307
dc.identifier.issn 1939-4586
dc.identifier.issue 10 en_US
dc.identifier.pmid 39259768
dc.identifier.scopus 2-s2.0-85205274410
dc.identifier.scopusquality Q3
dc.identifier.startpage br19 en_US
dc.identifier.uri https://doi.org/10.1091/mbc.E24-07-0307
dc.identifier.volume 35 en_US
dc.identifier.wos WOS:001330255100014
dc.identifier.wosquality Q3
dc.language.iso en en_US
dc.publisher Amer Soc Cell Biology en_US
dc.relation.ispartof Molecular biology of the cell en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 2
dc.subject [No Keyword Available] en_US
dc.title The Gtp-Tubulin Cap Is Not the Determinant of Microtubule End Stability in Cells en_US
dc.type Article en_US
dc.wos.citedbyCount 3
dspace.entity.type Publication
relation.isAuthorOfPublication 741b0353-dafc-4324-9902-db4c68740bd5
relation.isAuthorOfPublication.latestForDiscovery 741b0353-dafc-4324-9902-db4c68740bd5
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