Thermal Phase Transitions of Agarose in Various Compositions: A Fluorescence Study

dc.contributor.authorKara, Selim
dc.contributor.authorArda, Ertan
dc.contributor.authorDolastir, Fahrettin
dc.contributor.authorPekcan, Önder
dc.date.accessioned2019-06-27T08:04:38Z
dc.date.available2019-06-27T08:04:38Z
dc.date.issued2011
dc.departmentFakülteler, Mühendislik ve Doğa Bilimleri Fakültesi, Biyoinformatik ve Genetik Bölümüen_US
dc.description.abstractThe effect of agarose content on thermal phase transitions of the agarose gels was investigated by using Steady State Fluorescence (SSF) method. Scattered light I(sc) and fluorescence intensity I(fl) were monitored against temperature during heating and cooling processes to investigate phase transitions. Two regions were observed during the heating and cooling processes. At the high temperature region double helix to coil (h-c) transition took place. However during the cooling process coil to double helix (c-h) transitions occurred at low temperature region. Transition energies were determined using the Arrhenius treatment and found to be strongly correlated with the agarose content in the gel system. Transition temperatures were determined from the derivative of the sigmoidal transition paths and found to be increased by increasing agarose content in both cases.en_US]
dc.identifier.citation5
dc.identifier.doi10.1007/s10895-011-0883-6en_US
dc.identifier.endpage1877
dc.identifier.issn1053-0509en_US
dc.identifier.issn1053-0509
dc.identifier.issue5
dc.identifier.pmid21451985en_US
dc.identifier.scopus2-s2.0-80052971517en_US
dc.identifier.scopusqualityN/A
dc.identifier.startpage1871en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12469/968
dc.identifier.volume21en_US
dc.identifier.wosWOS:000296475900007en_US
dc.identifier.wosqualityN/A
dc.institutionauthorPekcan, Önderen_US
dc.language.isoenen_US
dc.publisherSpringer/Plenum Publishersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFluorescenceen_US
dc.subjectAgaroseen_US
dc.subjectPhase transitionen_US
dc.subjectGelsen_US
dc.subjectScatteringen_US
dc.titleThermal Phase Transitions of Agarose in Various Compositions: A Fluorescence Studyen_US
dc.typeArticleen_US
dspace.entity.typePublication

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