Thermal Phase Transitions of Agarose in Various Compositions: A Fluorescence Study

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Date

2011

Authors

Kara, Selim
Arda, Ertan
Dolastir, Fahrettin
Pekcan, Önder

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Publisher

Springer/Plenum Publishers

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Abstract

The effect of agarose content on thermal phase transitions of the agarose gels was investigated by using Steady State Fluorescence (SSF) method. Scattered light I(sc) and fluorescence intensity I(fl) were monitored against temperature during heating and cooling processes to investigate phase transitions. Two regions were observed during the heating and cooling processes. At the high temperature region double helix to coil (h-c) transition took place. However during the cooling process coil to double helix (c-h) transitions occurred at low temperature region. Transition energies were determined using the Arrhenius treatment and found to be strongly correlated with the agarose content in the gel system. Transition temperatures were determined from the derivative of the sigmoidal transition paths and found to be increased by increasing agarose content in both cases.

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Keywords

Fluorescence, Agarose, Phase transition, Gels, Scattering

Turkish CoHE Thesis Center URL

Citation

5

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N/A

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N/A

Source

Volume

21

Issue

5

Start Page

1871

End Page

1877