Electrical activity regulates AChR gene expression via JNK PKC zeta and Sp1 in skeletal chick muscle

dc.contributor.authorAltiok, Nedret
dc.contributor.authorChangeux, Jean-Pierre
dc.date.accessioned2019-06-27T08:01:05Z
dc.date.available2019-06-27T08:01:05Z
dc.date.issued2001
dc.description.abstractElectrical activity of myotubes represses nicotinic acetylcholine receptor (AChR) gene expression. This effect is mimicked by okadaic acid and blocked by tetrodotoxin (TTX) or staurosporine in cultured myocytes [Altiok et al. EMBO J. 16 (1997) 717-725]. In this study we investigated the mechanism of this repression. We show that addition of exogenous phospholipase D (PLD) and C inhibits AChR expression in a manner which parallels that of okadaic acid. Furthermore okadaic acid caused an increase of the threonine phosphorylation of protein kinase C zeta (PKC zeta) and activator of transcription factor (ATF2) and a decrease of the phosphorylation of Sp1. All these effects were reversed by staurosporine and TTX also abolished ATF2 phosphorylation. These data reveal a possible involvement of PLD c-jun N-terminal kinase PKC zeta and Sp1 in the repression of AChR genes by electrical activity. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.en_US]
dc.identifier.citation11
dc.identifier.doi10.1016/S0014-5793(00)02311-5en_US
dc.identifier.endpage338
dc.identifier.issn0014-5793en_US
dc.identifier.issn0014-5793
dc.identifier.issue3
dc.identifier.pmid11163354en_US
dc.identifier.scopus2-s2.0-0035808272en_US
dc.identifier.scopusqualityQ1
dc.identifier.startpage333en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12469/246
dc.identifier.urihttps://doi.org/10.1016/S0014-5793(00)02311-5
dc.identifier.volume487en_US
dc.identifier.wosWOS:000166441100005en_US
dc.identifier.wosqualityQ1
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.journalFebs Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectc-jun N-terminal kinaseen_US
dc.subjectPhospholipase Den_US
dc.subjectProtein kinase Cen_US
dc.subjectSkeletal muscleen_US
dc.subjectSp1en_US
dc.titleElectrical activity regulates AChR gene expression via JNK PKC zeta and Sp1 in skeletal chick muscleen_US
dc.typeArticleen_US
dspace.entity.typePublication

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